A novel DNA helicase from calf thymus.
نویسندگان
چکیده
منابع مشابه
Four different DNA helicases from calf thymus.
Using a strand displacement assay we have followed DNA helicase activities during the simultaneous isolation of several enzymes from calf thymus such as DNA polymerases alpha, delta, and epsilon, proliferating cell nuclear antigen, and replication factor A. Thus we were able to discriminate and isolate four different DNA helicases called A, B, C, and D. DNA helicase A is identical with the enzy...
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A new gene (POLL), has been identified encoding the novel DNA polymerase lambda and mapped to mouse chromosome 19 and at human chromosome 10. DNA polymerase lambda contains all the critical residues involved in DNA binding, nucleotide binding, nucleotide selection, and catalysis of DNA polymerization and has been assigned to family X based on sequence homology with polymerase beta, lambda, mu, ...
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Structural and functional differentiation of chro matin implies the search for the methods of isolation and characterization of DNA bound with chromatin fractions. Preliminary fractionation of chromatin fol lowed by the isolation of DNA from these fractions are the methods frequently used. The isolation procedure put forward by Strazhevskaya and Struchkov [1] makes it possible to isolate DNA ...
متن کاملGlutaredoxin from Calf Thymus PURIFICATION
The protein glutaredoxin, required for GSH-dependent ribonucleotide reduction, has been purified to homogeneity from calf thymus. The preparative method consisted of ammonium sulfate precipitation and three chromatography steps on DEAEkellulose, Sephadex G-50, and CM-Sepharose. Calf thymus glutaredoxin was assayed on the basis of its inherent GSH-disulfide transhydrogenase activity. Glutaredoxi...
متن کاملDeoxycytidine kinase from calf thymus.
Kinetic constants were determined for 34 nucleoside substrates of deoxycytidine kinase (EC 2.7.1.74) from calf thymus. Substrate efficiency was assessed by the ratio of V,,,,. to K,. Inhibition constants were determined for 61 nonsubstrate nucleosides or nucleoside analogues. The enzyme was relatively specific for the pentose moiety of nucleoside substrates. P-D-2’-Deoxyribonucleosides were mor...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)42259-4